Hemoglobinometry

 

Hemoglobin which is red color oxygen carrier,  to measure its percentage. If there is less hemoglobin it happens aneamia.

Materials-
Sahli's hemoglobinometer,
Pipette
Distilled water
Glass rod
Needle
In matching tube upto 2% mark - n/10 hcl fill.
By bleaking needle take blood out and keep in Pipette up to 20 mark.
Keep this blood in matching tube and shake frequently for 5-10 minutes until it get good mixture.
After this matching tube is kept in middle of the stand by which prefixed रंगदार नलोकाओं के साथ उसकी तुलना कि जासके . Now keep distilled water on it by drop by drop and do तुलना of the mixture.
Till the number of matching tube blood reaches there hemoglibin %age is mentioned.

Precaution:
1) There should not be air bubble when blood ti mixed in pipette
2 Mixture
3) Color
4) Pipette should be washed  by distilled water and dry it.

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